Hotspot

rps11

SSC · 114391-114762 · 372 bp

Pi 0.00806

MarkerSeek score 46.60

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: SSC
length_bp: 372
variable_sites: 3
pi: 0.00806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	GAACCCCTTCCAAAAGAGCA	CTAGAACTCGACGAGGGAGT	50.0	55.0	58.0	58.0	781 bp	1.0	80.3
rps11_p2	ATCTTCTCGAACCCCTTCCA	CTAGAACTCGACGAGGGAGT	50.0	55.0	58.0	58.0	789 bp	1.0	79.9
rps11_p3	GAACCCCTTCCAAAAGAGCA	TCGTCATGGCAGAGTTCAAG	50.0	50.0	58.0	57.9	1197 bp	1.0	79.7
rps11_p4	ATCTTCTCGAACCCCTTCCA	TCGTCATGGCAGAGTTCAAG	50.0	50.0	58.0	57.9	1205 bp	1.0	79.3
rps11_p5	GAACCCCTTCCAAAAGAGCA	TGGGAACACGCGTAAAAGAT	50.0	45.0	58.0	57.8	979 bp	1.0	79.1