Hotspot

rpl9

SSC · 100188-100655 · 468 bp

Pi 0.00641

MarkerSeek score 52.00

Primer available yes

Label rpl9

Feature Metadata

label_name: rpl9
feature_type: gene
region: SSC
length_bp: 468
variable_sites: 3
pi: 0.00641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl9_p1	AGGACTTGAACCTACGACCA	TTTCCCATTGAAGGTCGTCC	50.0	50.0	58.0	57.8	1576-1578 bp	1.0	78.8
rpl9_p2	CTCCCCAGGTAGGACTTGAA	TTTCCCATTGAAGGTCGTCC	55.0	50.0	58.0	57.8	1586-1588 bp	1.0	78.4
rpl9_p3	CGTTCTGGTTGTCCAATTGC	TTTCCCATTGAAGGTCGTCC	50.0	50.0	57.9	57.8	1824-1826 bp	1.0	78.3
rpl9_p4	ATCTCCCCAGGTAGGACTTG	TTTCCCATTGAAGGTCGTCC	55.0	50.0	57.8	57.8	1588-1590 bp	1.0	77.3
rpl9_p5	CCGATTGCTCTACCACTGAG	TTTCCCATTGAAGGTCGTCC	55.0	50.0	57.8	57.8	1544-1546 bp	1.0	77.1