Hotspot

rpl36

SSC · 115211-115324 · 114 bp

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Pi 0.0175
MarkerSeek score 62.60
Primer available yes
Label rpl36

Feature Metadata

label_name
rpl36
feature_type
gene
region
SSC
length_bp
114
variable_sites
2
pi
0.0175
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36_p1 AGAAGCCTTTTCAGCTGCTT CGAGTAAATCAGGCAGGAGT 45.0 50.0 58.0 57.0 1338 bp 1.0 72.5
rpl36_p2 ACTCCCTCGTCGAGTTCTAG CGAGTAAATCAGGCAGGAGT 55.0 50.0 58.0 57.0 1073 bp 1.0 72.3
rpl36_p3 GCACGAATAGCAGCTTCTCT CGAGTAAATCAGGCAGGAGT 50.0 50.0 58.1 57.0 1424 bp 1.0 72.1
rpl36_p4 AGAAGCCTTTTCAGCTGCTT ACCACTTCGAGTAAATCAGGC 45.0 47.6 58.0 58.0 1345 bp 1.0 72.0
rpl36_p5 AGAAGCCTTTTCAGCTGCTT CCACTTCGAGTAAATCAGGCA 45.0 47.6 58.0 58.0 1344 bp 1.0 72.0