Hotspot

rpl2

SSC · 122135-122962 · 828 bp

Back to Fucus

Pi 0.00484
MarkerSeek score 61.50
Primer available yes
Label rpl2

Feature Metadata

label_name
rpl2
feature_type
gene
region
SSC
length_bp
828
variable_sites
4
pi
0.00484
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2_p1 AGGGCCTTTACGAAAAGATCT GGCGATTTATTGGTATTCGAC 42.9 42.9 57.0 55.2 1029 bp 1.0 40.3
rpl2_p2 GCCTTTACGAAAAGATCTTGCC GGCGATTTATTGGTATTCGAC 45.5 42.9 58.2 55.2 1026 bp 1.0 40.3
rpl2_p3 GGCCTTTACGAAAAGATCTTGC GGCGATTTATTGGTATTCGAC 45.5 42.9 58.2 55.2 1027 bp 1.0 40.3
rpl2_p4 AAGGGCCTTTACGAAAAGATCT GGCGATTTATTGGTATTCGAC 40.9 42.9 57.7 55.2 1030 bp 1.0 40.3
rpl2_p5 AGGGCCTTTACGAAAAGATCTT GGCGATTTATTGGTATTCGAC 40.9 42.9 57.7 55.2 1029 bp 1.0 40.3