Hotspot

psaJ

LSC · 68561-68689 · 129 bp

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Pi 0.0391
MarkerSeek score 75.70
Primer available yes
Label psaJ

Feature Metadata

label_name
psaJ
feature_type
gene
region
LSC
length_bp
129
variable_sites
5
pi
0.0391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ_p1 GGCGTACCTTCATTTGTTGC GAAGATGGGATCACGCTGTT 50.0 50.0 58.0 58.0 936-939 bp 1.0 80.1
psaJ_p2 AAAAATCCCGACTCGTGAGG GAAGATGGGATCACGCTGTT 50.0 50.0 57.9 58.0 883-886 bp 1.0 79.5
psaJ_p3 GGCGTACCTTCATTTGTTGC TATTTGGCCCATTTCCGCTT 50.0 45.0 58.0 58.1 758-761 bp 1.0 79.3
psaJ_p4 GGCGTACCTTCATTTGTTGC TTATTTGGCCCATTTCCGCT 50.0 45.0 58.0 58.1 759-762 bp 1.0 79.3
psaJ_p5 ATCCCGACTCGTGAGGTTAA GAAGATGGGATCACGCTGTT 50.0 50.0 58.2 58.0 879-882 bp 1.0 79.1