Hotspot

ilvH

LSC · 47083-47733 · 651 bp

Pi 0.00461

MarkerSeek score 47.20

Primer available yes

Label ilvH

Feature Metadata

label_name: ilvH
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 3
pi: 0.00461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ilvH_p1	GAAATGTTGCCAGGAAACGG	TTTTACGGTGCAGAGACTCG	50.0	50.0	57.9	57.9	1595-1601 bp	1.0	79.7
ilvH_p2	GGAAATGTTGCCAGGAAACG	TTTTACGGTGCAGAGACTCG	50.0	50.0	57.9	57.9	1596-1602 bp	1.0	79.7
ilvH_p3	GAAATGTTGCCAGGAAACGG	GAAAAGGCAGGTAGCTGGTT	50.0	50.0	57.9	58.1	1199-1205 bp	1.0	79.6
ilvH_p4	GGAAATGTTGCCAGGAAACG	GAAAAGGCAGGTAGCTGGTT	50.0	50.0	57.9	58.1	1200-1206 bp	1.0	79.6
ilvH_p5	GAAATGTTGCCAGGAAACGG	CCTCCGCTCCCAATTGTAAA	50.0	50.0	57.9	57.9	1442-1448 bp	1.0	79.2