Hotspot

ORF501

LSC · 66984-68489 · 1506 bp

Back to Fucus

Pi 0.00133
MarkerSeek score 54.30
Primer available yes
Label ORF501

Feature Metadata

label_name
ORF501
feature_type
gene
region
LSC
length_bp
1506
variable_sites
2
pi
0.00133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF501_p1 TGGGGGATTTGGACAAACTG GAAGATGGGATCACGCTGTT 50.0 50.0 58.0 58.0 2324-2334 bp 1.0 80.0
ORF501_p2 CCAGATTTATCCAACGGGGG GAAGATGGGATCACGCTGTT 55.0 50.0 58.0 58.0 2549-2559 bp 1.0 79.9
ORF501_p3 GTGGTCGTGGCCAGATTTAT GAAGATGGGATCACGCTGTT 50.0 50.0 58.0 58.0 2559-2569 bp 1.0 79.8
ORF501_p4 TGGCCAGATTTATCCAACGG GAAGATGGGATCACGCTGTT 50.0 50.0 57.9 58.0 2552-2562 bp 1.0 79.7
ORF501_p5 GGTCCGGTTTCTGGTGATTT GAAGATGGGATCACGCTGTT 50.0 50.0 58.1 58.0 2674-2684 bp 1.0 79.3