Hotspot

psbH-ftsH

SSC · 107105-107166 · 62 bp

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Pi 0.0167
MarkerSeek score 65.10
Primer available yes
Label psbH-ftsH

Feature Metadata

label_name
psbH-ftsH
feature_type
igs
region
SSC
length_bp
62
variable_sites
1
pi
0.0167
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH-ftsH_p1 TGTTGATTGGCAAAGTTTAGGT TGAGACAATTGATGGGAAAGA 36.4 38.1 56.7 54.8 176-178 bp 1.0 41.4
psbH-ftsH_p2 TGTTGATTGGCAAAGTTTAGGT TGAGACAATTGATGGGAAAGAA 36.4 36.4 56.7 55.6 176-178 bp 1.0 41.4
psbH-ftsH_p3 TGTTGATTGGCAAAGTTTAGGT TTGAGACAATTGATGGGAAAGA 36.4 36.4 56.7 55.6 177-179 bp 1.0 41.3
psbH-ftsH_p4 TGTTGATTGGCAAAGTTTAGGT TCAAATTGAGACAATTGATGGGA 36.4 34.8 56.7 56.4 182-184 bp 1.0 41.3
psbH-ftsH_p5 TGATGTAGATGTTGATTGGCA TGAGACAATTGATGGGAAAGA 38.1 38.1 55.3 54.8 185-187 bp 1.0 41.3