Hotspot

trnT(tgt)-psbC

SSC · 96864-97110 · 247 bp

Back to Fucus

Pi 0.0122
MarkerSeek score 51.60
Primer available yes
Label trnT(tgt)-psbC

Feature Metadata

label_name
trnT(tgt)-psbC
feature_type
igs
region
SSC
length_bp
247
variable_sites
3
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(tgt)-psbC_p1 TTCAAGTCCTTTCACCAGCT TGTCCTTTCAATGCGTCCAA 45.0 45.0 57.0 58.0 296 bp 1.0 72.6
trnT(tgt)-psbC_p2 GTTCAAGTCCTTTCACCAGC TGTCCTTTCAATGCGTCCAA 50.0 45.0 56.9 58.0 297 bp 1.0 72.3
trnT(tgt)-psbC_p3 TTCAAGTCCTTTCACCAGCT CGACAAACAGAAGCTGTCCT 45.0 50.0 57.0 58.1 310 bp 1.0 71.8
trnT(tgt)-psbC_p4 TTCAAGTCCTTTCACCAGCT TCGACAAACAGAAGCTGTCC 45.0 50.0 57.0 58.1 311 bp 1.0 71.7
trnT(tgt)-psbC_p5 TTCAAGTCCTTTCACCAGCT ATTAGTGGGTCATTGGTGGC 45.0 50.0 57.0 57.9 380 bp 1.0 71.5