Hotspot

ycf3-atpB

LSC · 63077-63198 · 122 bp

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Pi 0.0246
MarkerSeek score 66.60
Primer available yes
Label ycf3-atpB

Feature Metadata

label_name
ycf3-atpB
feature_type
igs
region
LSC
length_bp
122
variable_sites
3
pi
0.0246
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-atpB_p1 GTACAACAGGCCGACTTTCA ACTGCATCAATTACTGGACCA 50.0 42.9 58.1 57.3 216 bp 1.0 66.3
ycf3-atpB_p2 ACTGGTTACGTACAACAGGC ACTGCATCAATTACTGGACCA 50.0 42.9 57.8 57.3 225 bp 1.0 66.0
ycf3-atpB_p3 ACAACAGGCCGACTTTCAAA ACTGCATCAATTACTGGACCA 45.0 42.9 58.2 57.3 214 bp 1.0 65.4
ycf3-atpB_p4 GTACAACAGGCCGACTTTCA AACTGCATCAATTACTGGACCA 50.0 40.9 58.1 58.0 217 bp 1.0 63.8
ycf3-atpB_p5 GTACAACAGGCCGACTTTCA ACTGCATCAATTACTGGACCAA 50.0 40.9 58.1 58.0 216 bp 1.0 63.8