Hotspot

rpl20-ilvH

LSC · 47007-47082 · 76 bp

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Pi 0.105
MarkerSeek score 81.00
Primer available yes
Label rpl20-ilvH

Feature Metadata

label_name
rpl20-ilvH
feature_type
igs
region
LSC
length_bp
76
variable_sites
8
pi
0.105
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-ilvH_p1 AGCGATTTTAGACCCATTAGCT ACACGTATTAATCCCTCAGAATCT 40.9 37.5 57.6 57.3 184 bp 1.0 43.5
rpl20-ilvH_p2 AGCGATTTTAGACCCATTAGCT ACACGTATTAATCCCTCAGA 40.9 40.0 57.6 53.2 184 bp 1.0 43.5
rpl20-ilvH_p3 AGCGATTTTAGACCCATTAGCT AACACGTATTAATCCCTCAGA 40.9 38.1 57.6 54.1 185 bp 1.0 43.5
rpl20-ilvH_p4 AGCGATTTTAGACCCATTAGCT ACACGTATTAATCCCTCAGAA 40.9 38.1 57.6 54.1 184 bp 1.0 43.5
rpl20-ilvH_p5 AGCGATTTTAGACCCATTAGCT AACACGTATTAATCCCTCAGAA 40.9 36.4 57.6 54.9 185 bp 1.0 43.5