Hotspot

psbI-trnG(gcc)

LSC · 17627-17764 · 138 bp

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Pi 0.0073
MarkerSeek score 43.50
Primer available yes
Label psbI-trnG(gcc)

Feature Metadata

label_name
psbI-trnG(gcc)
feature_type
igs
region
LSC
length_bp
138
variable_sites
1
pi
0.0073
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnG(gcc)_p1 TGATCCATCAAGAAATCCAA CTCTACCACTGAGCTACACTC 35.0 52.4 52.1 57.0 201 bp 1.0 40.4
psbI-trnG(gcc)_p2 TGATCCATCAAGAAATCCAAA CTCTACCACTGAGCTACACTC 33.3 52.4 53.1 57.0 201 bp 1.0 40.4
psbI-trnG(gcc)_p3 TGATCCATCAAGAAATCCAA TCTACCACTGAGCTACACTC 35.0 50.0 52.1 55.7 200 bp 1.0 40.4
psbI-trnG(gcc)_p4 TGATCCATCAAGAAATCCAA CTCTACCACTGAGCTACACT 35.0 50.0 52.1 55.7 201 bp 1.0 40.4
psbI-trnG(gcc)_p5 TGATCCATCAAGAAATCCAAA TCTACCACTGAGCTACACTC 33.3 50.0 53.1 55.7 200 bp 1.0 40.4