Hotspot

rps15

Genome · 103585-103851 · 267 bp

Back to Frullania

Pi 0.015

MarkerSeek score 46.30

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: Genome
length_bp: 267
variable_sites: 4
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TTTGAGCACCGATATCAGCC	CAGAGCATCGTTTACCTCGA	50.0	50.0	58.0	57.4	1297-1308 bp	1.0	77.5
rps15_p2	TTTGAGCACCGATATCAGCC	TCAGAGCATCGTTTACCTCG	50.0	50.0	58.0	57.4	1298-1309 bp	1.0	77.5
rps15_p3	GCCGTAAAACACCATGCATC	CAGAGCATCGTTTACCTCGA	50.0	50.0	58.1	57.4	994-1005 bp	1.0	77.5
rps15_p4	GCCGTAAAACACCATGCATC	TCAGAGCATCGTTTACCTCG	50.0	50.0	58.1	57.4	995-1006 bp	1.0	77.5
rps15_p5	GCACCGATATCAGCCATGAA	CAGAGCATCGTTTACCTCGA	50.0	50.0	58.1	57.4	1292-1303 bp	1.0	76.9