Hotspot

cemA

Genome · 61189-62193 · 1005 bp

Back to Frullania

Pi 0.0149

MarkerSeek score 53.00

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 1005
variable_sites: 15
pi: 0.0149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCTGGAACGTAGGTAGTGGG	GGAATACGATCAGACGGAGC	55.0	55.0	58.2	57.9	2317-2322 bp	1.0	79.7
cemA_p2	TGGAAACTAAAGCGGCTGAA	GGAATACGATCAGACGGAGC	45.0	55.0	57.7	57.9	2074-2079 bp	1.0	78.9
cemA_p3	TCTGGAACGTAGGTAGTGGG	TTCATAACCCTGTTGCGCAT	55.0	45.0	58.2	58.2	2078-2083 bp	1.0	78.9
cemA_p4	TCTGGAACGTAGGTAGTGGG	CCCTGTTGCGCATAAATTGG	55.0	50.0	58.2	58.1	2071 bp	0.5	58.1
cemA_p5	TCTGGAACGTAGGTAGTGGG	CCTGTTGCGCATAAATTGGG	55.0	50.0	58.2	58.1	2070 bp	0.5	58.1