Hotspot

rps15-ycf1-2

Genome · 103852-104006 · 155 bp

Back to Frullania

Pi 0.0694

MarkerSeek score 74.00

Primer available yes

Label rps15-ycf1-2

Feature Metadata

label_name: rps15-ycf1-2
feature_type: igs
region: Genome
length_bp: 155
variable_sites: 10
pi: 0.0694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1-2_p1	CCACAGACCTCGTTGAGATG	AGACTTGCTTCGGGAAACAG	55.0	50.0	58.0	58.1	991-1002 bp	1.0	81.5
rps15-ycf1-2_p2	CCACAGACCTCGTTGAGATG	GACTTGCTTCGGGAAACAGA	55.0	50.0	58.0	58.1	990-1001 bp	1.0	81.5
rps15-ycf1-2_p3	TCCACAGACCTCGTTGAGAT	AGACTTGCTTCGGGAAACAG	50.0	50.0	58.1	58.1	992-1003 bp	1.0	81.0
rps15-ycf1-2_p4	TTTGAGCACCGATATCAGCC	AGACTTGCTTCGGGAAACAG	50.0	50.0	58.0	58.1	1524-1535 bp	1.0	80.7
rps15-ycf1-2_p5	TTTGAGCACCGATATCAGCC	GACTTGCTTCGGGAAACAGA	50.0	50.0	58.0	58.1	1523-1534 bp	1.0	80.6