Hotspot

trnP-UGG-psaJ

Genome · 65507-65724 · 218 bp

Back to Frullania

Pi 0.0642

MarkerSeek score 77.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 218
variable_sites: 14
pi: 0.0642
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TACCAAACTGCGCTACATCC	GCTAAAACGGGTGCTGTAGA	50.0	50.0	58.0	57.9	284-285 bp	1.0	83.1
trnP-UGG-psaJ_p2	TACCAAACTGCGCTACATCC	AGCTAAAACGGGTGCTGTAG	50.0	50.0	58.0	57.9	285-286 bp	1.0	83.0
trnP-UGG-psaJ_p3	TACCAAACTGCGCTACATCC	AGTAGCTAAAACGGGTGCTG	50.0	50.0	58.0	57.9	288-289 bp	1.0	83.0
trnP-UGG-psaJ_p4	GGGATGACAGGATTCGAACC	GCTAAAACGGGTGCTGTAGA	55.0	50.0	58.0	57.9	334-335 bp	1.0	82.3
trnP-UGG-psaJ_p5	GGGATGACAGGATTCGAACC	AGCTAAAACGGGTGCTGTAG	55.0	50.0	58.0	57.9	335-336 bp	1.0	82.2