Hotspot

atpB-rbcL

Genome · 56256-56935 · 680 bp

Back to Frullania

Pi 0.0165

MarkerSeek score 69.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 680
variable_sites: 11
pi: 0.0165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCGACATCAAGTACTGGACC	GTCTCCGTTTGTGGTGACAT	55.0	50.0	58.4	58.1	785-795 bp	1.0	78.5
atpB-rbcL_p2	ACAAGTGTAGAAGCCCCAAG	GTCTCCGTTTGTGGTGACAT	50.0	50.0	57.4	58.1	731-741 bp	1.0	77.0
atpB-rbcL_p3	AGCGACATCAAGTACTGGAC	GTCTCCGTTTGTGGTGACAT	50.0	50.0	57.3	58.1	786-796 bp	1.0	76.1
atpB-rbcL_p4	CAAGTGTAGAAGCCCCAAGA	GTCTCCGTTTGTGGTGACAT	50.0	50.0	57.1	58.1	730-740 bp	1.0	74.7
atpB-rbcL_p5	CGACATCAAGTACTGGACCA	GTCTCCGTTTGTGGTGACAT	50.0	50.0	57.0	58.1	784-794 bp	1.0	73.1