Hotspot

trnS-GGA-rps4

Genome · 49750-49824 · 75 bp

Back to Frullania

Pi 0.0704

MarkerSeek score 80.10

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 75
variable_sites: 5
pi: 0.0704
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTTTGTCTACCGAGGGTTCG	ACACAGTTGCAAGGTTCTGT	50.0	45.0	57.6	57.9	210-213 bp	1.0	78.8
trnS-GGA-rps4_p2	GGGTTCGAATCCCTCTCTTT	ACACAGTTGCAAGGTTCTGT	50.0	45.0	56.9	57.9	197-200 bp	1.0	73.9
trnS-GGA-rps4_p3	GGGTTCGAATCCCTCTCTTTC	ACACAGTTGCAAGGTTCTGT	52.4	45.0	58.1	57.9	197-200 bp	1.0	73.9
trnS-GGA-rps4_p4	GGTTCGAATCCCTCTCTTTCC	ACACAGTTGCAAGGTTCTGT	52.4	45.0	58.1	57.9	196-199 bp	1.0	73.9
trnS-GGA-rps4_p5	TTTTTGTCTACCGAGGGTTCG	ACACAGTTGCAAGGTTCTGT	47.6	45.0	58.2	57.9	211-214 bp	1.0	72.4