Hotspot

trnD-GUC-trnY-GUA

Genome · 37147-37303 · 157 bp

Back to Frullania

Pi 0.0536
MarkerSeek score 72.50
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
Genome
length_bp
157
variable_sites
6
pi
0.0536
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.71
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGCGGTGCTCTAACCTATTG CTGGTTCAAATCCAGCTCGA 55.0 50.0 58.4 57.9 169-213 bp 1.0 82.4
trnD-GUC-trnY-GUA_p2 GGCGGTGCTCTAACCTATTG CCTACGCTGGTTCAAATCCA 55.0 50.0 58.4 57.9 175-219 bp 1.0 82.2
trnD-GUC-trnY-GUA_p3 GGCGGTGCTCTAACCTATTG TGGTTCAAATCCAGCTCGAC 55.0 50.0 58.4 58.2 168-212 bp 1.0 81.7
trnD-GUC-trnY-GUA_p4 CAACTTCCGTCTTGACAGGG CTGGTTCAAATCCAGCTCGA 55.0 50.0 58.5 57.9 187-231 bp 1.0 81.6
trnD-GUC-trnY-GUA_p5 CAACTTCCGTCTTGACAGGG CCTACGCTGGTTCAAATCCA 55.0 50.0 58.5 57.9 193-237 bp 1.0 81.5