Hotspot

ndhG-ndhI

SSC · 116418-116811 · 394 bp

Back to Fritillaria

Pi 0.0139

MarkerSeek score 64.20

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 48
pi: 0.0139
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TATTCCCAGGCCCCCAAATA	TCAAACAGTTATGAATTCGACTCA	50.0	33.3	58.1	56.7	275-548 bp	1.0	51.9
ndhG-ndhI_p2	TCCCAGGCCCCCAAATATAA	TCAAACAGTTATGAATTCGACTCA	50.0	33.3	58.1	56.7	272-545 bp	1.0	51.9
ndhG-ndhI_p3	TTCCCAGGCCCCCAAATATA	TCAAACAGTTATGAATTCGACTCA	50.0	33.3	58.1	56.7	273-546 bp	1.0	51.9
ndhG-ndhI_p4	CCCAGGCCCCCAAATATAAG	TCAAACAGTTATGAATTCGACTCA	55.0	33.3	57.7	56.7	271-544 bp	1.0	51.9
ndhG-ndhI_p5	TCCCAGGCCCCCAAATATAA	CAAACAGTTATGAATTCGACTCA	50.0	34.8	58.1	55.3	271-544 bp	1.0	51.9