Hotspot

rpl32-trnL-UAG

SSC · 111076-111818 · 743 bp

Back to Fritillaria

Pi 0.0232
MarkerSeek score 74.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
743
variable_sites
110
pi
0.0232
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACTGGGCATTCAAAAAGTTT ACTGCTTCCTAAGAACAGCG 35.0 50.0 54.1 57.9 729-885 bp 1.0 60.4
rpl32-trnL-UAG_p2 ACTGGGCATTCAAAAAGTTT CTGCTTCCTAAGAACAGCGT 35.0 50.0 54.1 57.9 728-884 bp 1.0 60.4
rpl32-trnL-UAG_p3 ACTGGGCATTCAAAAAGTTT GCTTCCTAAGAACAGCGTGT 35.0 50.0 54.1 58.2 726-882 bp 1.0 59.5
rpl32-trnL-UAG_p4 ACTGGGCATTCAAAAAGTTT TGCTTCCTAAGAACAGCGTG 35.0 50.0 54.1 58.2 727-883 bp 1.0 59.4
rpl32-trnL-UAG_p5 ACTGGGCATTCAAAAAGTTTT CTGCTTCCTAAGAACAGCGT 33.3 50.0 55.0 57.9 728-884 bp 1.0 59.3