Hotspot

rpl36-rps8

LSC · 77345-77803 · 459 bp

Back to Fritillaria

Pi 0.0159

MarkerSeek score 63.20

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 459
variable_sites: 44
pi: 0.0159
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	CACTATAATCCGTCCACGCC	TCGGGGGAGAAATTTTGTGT	55.0	45.0	58.2	57.3	560-574 bp	1.0	80.9
rpl36-rps8_p2	ACTATAATCCGTCCACGCCT	TCGGGGGAGAAATTTTGTGT	50.0	45.0	58.3	57.3	559-573 bp	1.0	80.3
rpl36-rps8_p3	CACTATAATCCGTCCACGCC	TCGGGGGAGAAATTTTGTGTT	55.0	42.9	58.2	58.0	560-574 bp	1.0	77.9
rpl36-rps8_p4	ACTATAATCCGTCCACGCCT	TCGGGGGAGAAATTTTGTGTT	50.0	42.9	58.3	58.0	559-573 bp	1.0	77.3
rpl36-rps8_p5	CACTATAATCCGTCCACGCC	ATCGGGGGAGAAATTTTGTGT	55.0	42.9	58.2	57.8	561-575 bp	1.0	76.9