Hotspot

ycf4-cemA

LSC · 57995-58728 · 734 bp

Back to Fritillaria

Pi 0.0225

MarkerSeek score 63.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 96
pi: 0.0225
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGGTGTACAATTGCGTGGAA	ACAAGCGAAGGAGATACACG	45.0	50.0	58.0	57.7	1281-1751 bp	1.0	88.3
ycf4-cemA_p2	TTTCGTTGGGGATTTCCTGG	ACAAGCGAAGGAGATACACG	50.0	50.0	58.1	57.7	1209-1679 bp	1.0	88.2
ycf4-cemA_p3	GCGTGGAATGTAGGTAGTGG	ACAAGCGAAGGAGATACACG	55.0	50.0	58.1	57.7	1269-1739 bp	1.0	88.1
ycf4-cemA_p4	AACGGGGTCTCGAAAAACAA	ACAAGCGAAGGAGATACACG	45.0	50.0	58.0	57.7	1483-1953 bp	1.0	87.8
ycf4-cemA_p5	GGAAATAAGAGGCCAAGGGG	ACAAGCGAAGGAGATACACG	55.0	50.0	57.9	57.7	1090-1560 bp	0.969697	87.2