Hotspot

ndhC-trnV-UAC

LSC · 48197-48933 · 737 bp

Back to Fritillaria

Pi 0.0125
MarkerSeek score 65.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
737
variable_sites
57
pi
0.0125
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGTGCTAAAACTCCGGAAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.4 57.8 849-870 bp 1.0 82.7
ndhC-trnV-UAC_p2 TTGGTGCTAAAACTCCGGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.4 57.8 850-871 bp 1.0 82.7
ndhC-trnV-UAC_p3 ATTGGTGCTAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.1 57.8 851-872 bp 1.0 80.9
ndhC-trnV-UAC_p4 TGGTGCTAAAACTCCGGAAA AGCCCAATGCAACAAAATGC 45.0 45.0 57.4 58.5 935-955 bp 1.0 80.4
ndhC-trnV-UAC_p5 TTGGTGCTAAAACTCCGGAA AGCCCAATGCAACAAAATGC 45.0 45.0 57.4 58.5 936-956 bp 1.0 80.4