Hotspot

trnT-UGU-trnL-UAA

LSC · 44107-44878 · 772 bp

Back to Fritillaria

Pi 0.0212

MarkerSeek score 73.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 102
pi: 0.0212
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	753-896 bp	1.0	89.0
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	785-928 bp	1.0	88.1
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	747-890 bp	1.0	87.7
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	785-928 bp	1.0	86.9
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	CAGGTTTCCATACCAAGGCT	55.0	50.0	57.9	57.5	787-930 bp	1.0	86.8