Hotspot

trnD-GUC-trnY-GUA

LSC · 28449-28752 · 304 bp

Back to Fritillaria

Pi 0.0231

MarkerSeek score 70.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 304
variable_sites: 46
pi: 0.0231
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGATCCTAGTTCGGGACTGA	TGGCAATATGTCTACGCTGG	55.0	50.0	57.9	57.8	415-458 bp	1.0	89.5
trnD-GUC-trnY-GUA_p2	GGATCCTAGTTCGGGACTGA	GGCAATATGTCTACGCTGGT	55.0	50.0	57.9	57.8	414-457 bp	1.0	89.5
trnD-GUC-trnY-GUA_p3	GGATCCTAGTTCGGGACTGA	CGGACTGTAAATTCGTTGGC	55.0	50.0	57.9	57.5	431-474 bp	1.0	87.3
trnD-GUC-trnY-GUA_p4	ATCGGATCCTAGTTCGGGAC	TGGCAATATGTCTACGCTGG	55.0	50.0	58.4	57.8	418-461 bp	1.0	87.0
trnD-GUC-trnY-GUA_p5	CATCGGATCCTAGTTCGGGA	TGGCAATATGTCTACGCTGG	55.0	50.0	58.4	57.8	419-462 bp	1.0	87.0