Hotspot

atpH-atpI

LSC · 12648-13533 · 886 bp

Back to Fritillaria

Pi 0.0137

MarkerSeek score 67.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 62
pi: 0.0137
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	895-988 bp	0.969697	83.6
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	896-989 bp	0.969697	82.5
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	910-1003 bp	0.969697	82.1
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.0	57.4	868-961 bp	0.909091	80.4
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.2	57.4	869-962 bp	0.909091	79.2