Hotspot

trnK-UUU-rps16

LSC · 3987-4717 · 731 bp

Back to Fritillaria

Pi 0.0189
MarkerSeek score 81.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
731
variable_sites
92
pi
0.0189
unique_haplotype_count
33
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCTCAACCCACAGTAACTGT 50.0 50.0 58.2 57.7 746-847 bp 1.0 88.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCTCAACCCACAGTAACTGT 50.0 50.0 58.2 57.7 745-846 bp 1.0 88.6
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CTTGAAAATGGCGCTCAACC 50.0 50.0 58.2 58.3 758-859 bp 1.0 88.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAATGGCGCTCAAC 50.0 50.0 58.2 58.3 758-859 bp 1.0 88.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAATGGCGCTCAACC 50.0 50.0 58.2 58.3 757-858 bp 1.0 88.3