Hotspot

ycf1

SSC · 125367-130991 · 5625 bp

Back to Fraxinus

Pi 0.00756

MarkerSeek score 53.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 205
pi: 0.00756
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAACATTAGTATCAGTTACAATTCT	ACCATTATCAACTGAAATTGTTG	26.9	30.4	54.8	53.8	186-189 bp	1.0	47.6
ycf1_p2	TGAACATTAGTATCAGTTACAATTCT	AACCATTATCAACTGAAATTGTTG	26.9	29.2	54.8	54.6	187-190 bp	1.0	47.6
ycf1_p3	TTGAACATTAGTATCAGTTACAATTCT	ACCATTATCAACTGAAATTGTTG	25.9	30.4	55.4	53.8	187-190 bp	1.0	47.6
ycf1_p4	TGAACATTAGTATCAGTTACAATTC	ACCATTATCAACTGAAATTGTTG	28.0	30.4	53.4	53.8	186-189 bp	0.958333	45.5
ycf1_p5	TTTGAACATTAGTATCAGTTACAA	ACCATTATCAACTGAAATTGTTG	25.0	30.4	52.4	53.8	188-191 bp	0.958333	45.5