Hotspot

rpl16

LSC · 83541-84824 · 1284 bp

Back to Fraxinus

Pi 0.00632

MarkerSeek score 46.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1284
variable_sites: 34
pi: 0.00632
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCCCAAGATTCATTTCTGTTGG	TCAATTGACCATTTGATATAATTGCT	45.5	26.9	57.5	56.1	1296-1342 bp	1.0	45.0
rpl16_p2	CCCCAAGATTCATTTCTGTTGG	TCAATTGACCATTTGATATAATTGC	45.5	28.0	57.5	54.8	1296-1342 bp	1.0	45.0
rpl16_p3	CCCCAAGATTCATTTCTGTTGG	CAATTGACCATTTGATATAATTGCT	45.5	28.0	57.5	54.8	1295-1341 bp	1.0	45.0
rpl16_p4	CCCCAAGATTCATTTCTGTTGG	TTCAATTGACCATTTGATATAATTGCT	45.5	25.9	57.5	56.7	1297-1343 bp	1.0	45.0
rpl16_p5	CCCCAAGATTCATTTCTGTTGG	ATTGACCATTTGATATAATTGCT	45.5	26.1	57.5	52.5	1293-1339 bp	1.0	45.0