Hotspot

trnL(UAG)-ccsA

SSC · 115860-115949 · 90 bp

Back to Fraxinus

Pi 0.0223
MarkerSeek score 54.50
Primer available yes
Label trnL(UAG)-ccsA

Feature Metadata

label_name
trnL(UAG)-ccsA
feature_type
igs
region
SSC
length_bp
90
variable_sites
6
pi
0.0223
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(UAG)-ccsA_p1 TAGACACGCTGCTCTTAGGA ACAATTGAAACGATCGAAAAAGA 50.0 30.4 57.9 55.7 213 bp 1.0 42.3
trnL(UAG)-ccsA_p2 AAATTGGTAGACACGCTGCT ACAATTGAAACGATCGAAAAAGA 45.0 30.4 57.8 55.7 220 bp 1.0 42.2
trnL(UAG)-ccsA_p3 GTAGACACGCTGCTCTTAGG ACAATTGAAACGATCGAAAAAGA 55.0 30.4 57.8 55.7 214 bp 1.0 42.2
trnL(UAG)-ccsA_p4 GAGTTCAAGACAAGCCGCTA ACAATTGAAACGATCGAAAAAGA 50.0 30.4 57.9 55.7 245 bp 1.0 42.0
trnL(UAG)-ccsA_p5 ACAAGCCGCTATGGTGAAAT ACAATTGAAACGATCGAAAAAGA 45.0 30.4 57.9 55.7 236 bp 1.0 42.0