Hotspot

rpl32-trnL(UAG)

SSC · 114918-115779 · 862 bp

Back to Fraxinus

Pi 0.0102

MarkerSeek score 57.30

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 862
variable_sites: 38
pi: 0.0102
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	TGGGACAGCGTTAAAAGCAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	983-1001 bp	1.0	83.5
rpl32-trnL(UAG)_p2	TGGGACAGCGTTAAAAGCAT	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	976-994 bp	1.0	83.1
rpl32-trnL(UAG)_p3	TGGGACAGCGTTAAAAGCAT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.1	57.8	982-1000 bp	1.0	82.9
rpl32-trnL(UAG)_p4	TGGGACAGCGTTAAAAGCAT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.1	57.8	981-999 bp	1.0	82.9
rpl32-trnL(UAG)_p5	TGGGACAGCGTTAAAAGCAT	GAGCAGCGTGTCTACCAATT	45.0	50.0	58.1	58.3	977-995 bp	1.0	82.3