Hotspot

atpB-rbcL

LSC · 56500-57279 · 780 bp

Back to Fraxinus

Pi 0.0143

MarkerSeek score 58.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 34
pi: 0.0143
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.0	58.3	898-920 bp	1.0	80.9
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	869-891 bp	1.0	80.3
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	940-962 bp	1.0	79.6
atpB-rbcL_p4	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.2	58.3	897-919 bp	1.0	79.2
atpB-rbcL_p5	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	916-938 bp	1.0	79.0