Hotspot

psaA-ycf3

LSC · 43514-44231 · 718 bp

Back to Fraxinus

Pi 0.00539

MarkerSeek score 58.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 17
pi: 0.00539
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	529-852 bp	1.0	83.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	50.0	45.0	57.9	57.7	602-925 bp	1.0	82.4
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	50.0	50.0	57.9	58.5	610-933 bp	1.0	80.9
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	554-877 bp	1.0	80.8
psaA-ycf3_p5	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	482-805 bp	1.0	80.8