Hotspot

trnM(CAU)-psbD

LSC · 33249-34560 · 1312 bp

Back to Fraxinus

Pi 0.00572
MarkerSeek score 56.10
Primer available yes
Label trnM(CAU)-psbD

Feature Metadata

label_name
trnM(CAU)-psbD
feature_type
igs
region
LSC
length_bp
1312
variable_sites
37
pi
0.00572
unique_haplotype_count
17
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM(CAU)-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1290-1475 bp 1.0 84.7
trnM(CAU)-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1293-1478 bp 1.0 84.5
trnM(CAU)-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1280-1465 bp 1.0 83.6
trnM(CAU)-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1283-1468 bp 1.0 83.5
trnM(CAU)-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1267-1452 bp 1.0 83.0