Hotspot

trnS(GCU)-trnG(GCC)

LSC · 9375-10078 · 704 bp

Back to Fraxinus

Pi 0.00582

MarkerSeek score 57.70

Primer available yes

Label trnS(GCU)-trnG(GCC)

Feature Metadata

label_name: trnS(GCU)-trnG(GCC)
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 24
pi: 0.00582
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnG(GCC)_p1	CAATCCGACGCTTTAGTCCA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	787-818 bp	1.0	67.0
trnS(GCU)-trnG(GCC)_p2	AATCCGACGCTTTAGTCCAC	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.0	56.6	786-817 bp	1.0	67.0
trnS(GCU)-trnG(GCC)_p3	ATCAACGGAACCGGAAAGAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	57.9	56.6	851-882 bp	1.0	66.3
trnS(GCU)-trnG(GCC)_p4	CATCAACGGAACCGGAAAGA	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	852-883 bp	1.0	65.5
trnS(GCU)-trnG(GCC)_p5	TCATCAACGGAACCGGAAAG	AGACCGAAAGACCCTTTAACT	50.0	42.9	58.2	56.6	853-884 bp	1.0	65.5