Hotspot

rps16-trnQ(UUG)

LSC · 6430-8073 · 1644 bp

Back to Fraxinus

Pi 0.00743

MarkerSeek score 56.60

Primer available yes

Label rps16-trnQ(UUG)

Feature Metadata

label_name: rps16-trnQ(UUG)
feature_type: igs
region: LSC
length_bp: 1644
variable_sites: 62
pi: 0.00743
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ(UUG)_p1	CGGATCTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.1	58.1	1504-1748 bp	1.0	85.6
rps16-trnQ(UUG)_p2	ATCTGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1501-1745 bp	1.0	85.6
rps16-trnQ(UUG)_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1490-1734 bp	1.0	85.2
rps16-trnQ(UUG)_p4	CCACAACGGATCTGTCCTTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.3	58.1	1510-1754 bp	1.0	84.1
rps16-trnQ(UUG)_p5	GGATCTGTCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1503-1747 bp	1.0	83.4