Hotspot

trnN-GUU-trnR-ACG

IRa · 129331-129791 · 461 bp

Back to Frankenia

Pi 0.0174

MarkerSeek score 65.80

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 461
variable_sites: 8
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	602-653 bp	1.0	79.3
trnN-GUU-trnR-ACG_p2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	553-604 bp	1.0	78.1
trnN-GUU-trnR-ACG_p3	TTCGAATCCTACCTGGGGAG	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.2	57.8	543-594 bp	1.0	77.7
trnN-GUU-trnR-ACG_p4	CCAATCTCCCGTTGTTCCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.3	57.8	610-661 bp	1.0	77.2
trnN-GUU-trnR-ACG_p5	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.7	57.8	557-608 bp	1.0	77.2