Hotspot

ndhG-ndhI

SSC · 118556-118863 · 308 bp

Back to Frankenia

Pi 0.0676

MarkerSeek score 87.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 308
variable_sites: 20
pi: 0.0676
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAAAGAAAAGGCAGCAA	TCAAATTGCTTTGGGTCGTT	45.0	40.0	58.2	56.4	523-528 bp	1.0	70.7
ndhG-ndhI_p2	TCCCAAAGAAAAGGCAGCAA	ATCAAATTGCTTTGGGTCGT	45.0	40.0	58.2	56.2	524-529 bp	1.0	68.9
ndhG-ndhI_p3	TCCCAAAGAAAAGGCAGCAA	TCAAATTGCTTTGGGTCGTTT	45.0	38.1	58.2	57.2	523-528 bp	1.0	68.7
ndhG-ndhI_p4	CCCAAAGAAAAGGCAGCAAA	TCAAATTGCTTTGGGTCGTT	45.0	40.0	57.4	56.4	522-527 bp	1.0	67.5
ndhG-ndhI_p5	TCCCAAAGAAAAGGCAGCAA	ATCAAATTGCTTTGGGTCGTT	45.0	38.1	58.2	57.0	524-529 bp	1.0	66.9