Hotspot

ndhD-psaC

SSC · 116914-117049 · 136 bp

Back to Frankenia

Pi 0.0444

MarkerSeek score 70.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 136
variable_sites: 6
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAAAACGAAAGAACCTGCGA	AGAGATGTGAATCTGCCTGC	45.0	50.0	57.0	57.7	290-291 bp	1.0	71.1
ndhD-psaC_p2	AGAAAACGAAAGAACCTGCG	AGAGATGTGAATCTGCCTGC	45.0	50.0	57.0	57.7	291-292 bp	1.0	71.0
ndhD-psaC_p3	GAAAACGAAAGAACCTGCGA	CAGAGGACTGTGTTGGTTGT	45.0	50.0	57.0	57.7	311-312 bp	1.0	70.9
ndhD-psaC_p4	AGAAAACGAAAGAACCTGCG	CAGAGGACTGTGTTGGTTGT	45.0	50.0	57.0	57.7	312-313 bp	1.0	70.8
ndhD-psaC_p5	GAAAACGAAAGAACCTGCGAA	AGAGATGTGAATCTGCCTGC	42.9	50.0	57.7	57.7	290-291 bp	1.0	68.7