Hotspot

ndhF-rpl32

SSC · 111766-112737 · 972 bp

Back to Frankenia

Pi 0.0154

MarkerSeek score 54.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 972
variable_sites: 15
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AATCCAAAGTCTCCGACACG	TCGCTTTCGAAAGAGATGCT	50.0	45.0	57.9	57.6	1662-1679 bp	1.0	77.4
ndhF-rpl32_p2	CCAAAGTCTCCGACACGATT	TCGCTTTCGAAAGAGATGCT	50.0	45.0	57.9	57.6	1659-1676 bp	1.0	77.4
ndhF-rpl32_p3	ACCACACATGAAGCGGAAAT	TCGCTTTCGAAAGAGATGCT	45.0	45.0	58.1	57.6	1844-1861 bp	1.0	77.3
ndhF-rpl32_p4	AAAAGACCACATACGCCGAA	TCGCTTTCGAAAGAGATGCT	45.0	45.0	57.8	57.6	1222-1239 bp	1.0	76.8
ndhF-rpl32_p5	AAAGACCACATACGCCGAAA	TCGCTTTCGAAAGAGATGCT	45.0	45.0	57.8	57.6	1221-1238 bp	1.0	76.8