Hotspot

rpl33-rps18

LSC · 66625-67240 · 616 bp

Back to Frankenia

Pi 0.0182
MarkerSeek score 57.10
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
616
variable_sites
11
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 ATCAAGCGGCATTTCGAGAT GTTCCCCGGAGAATGAACTC 45.0 55.0 58.0 58.0 1253-1254 bp 1.0 81.3
rpl33-rps18_p2 GGTTCATGGCCAAGGGTAAA GTTCCCCGGAGAATGAACTC 50.0 55.0 58.1 58.0 1335-1336 bp 1.0 81.0
rpl33-rps18_p3 AAAGGAGGTTCATGGCCAAG GTTCCCCGGAGAATGAACTC 50.0 55.0 58.1 58.0 1341-1342 bp 1.0 81.0
rpl33-rps18_p4 AAGGTCGATGGAGGACAAGA GTTCCCCGGAGAATGAACTC 50.0 55.0 58.1 58.0 1518-1528 bp 1.0 80.9
rpl33-rps18_p5 ATGCGTTGACATTCCCCTTT GTTCCCCGGAGAATGAACTC 45.0 55.0 58.1 58.0 1694-1705 bp 1.0 80.7