Hotspot

ndhC-trnV-UAC

LSC · 49603-50603 · 1001 bp

Back to Frankenia

Pi 0.018
MarkerSeek score 60.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1001
variable_sites
18
pi
0.018
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTGAAAGTAGAAATGCAA CGGTTCAAGTCCGTATAGCC 35.0 55.0 52.4 58.2 1111-1117 bp 1.0 41.3
ndhC-trnV-UAC_p2 TCCTGAAAGTAGAAATGCAAA CGGTTCAAGTCCGTATAGCC 33.3 55.0 53.3 58.2 1111-1117 bp 1.0 41.3
ndhC-trnV-UAC_p3 TCCTGAAAGTAGAAATGCAA GGTTCAAGTCCGTATAGCCC 35.0 55.0 52.4 57.8 1110-1116 bp 1.0 41.3
ndhC-trnV-UAC_p4 TCCTGAAAGTAGAAATGCAAA GGTTCAAGTCCGTATAGCCC 33.3 55.0 53.3 57.8 1110-1116 bp 1.0 41.3
ndhC-trnV-UAC_p5 TCCTGAAAGTAGAAATGCAAAA CGGTTCAAGTCCGTATAGCC 31.8 55.0 54.2 58.2 1111-1117 bp 1.0 41.3