Hotspot

trnT-GGU-psbD

LSC · 30537-31960 · 1424 bp

Back to Frankenia

Pi 0.00965

MarkerSeek score 68.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1424
variable_sites: 13
pi: 0.00965
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	TCTCCGTAACCAGTCATCCA	50.0	50.0	57.9	57.8	1496-1571 bp	1.0	82.0
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.3	57.8	1459-1534 bp	1.0	80.6
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	GGTCTCTCCGTAACCAGTCA	50.0	55.0	57.9	58.5	1500-1575 bp	1.0	80.2
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	AGCAATAAACCGGACCAACC	50.0	50.0	57.9	58.5	1534-1609 bp	1.0	80.1
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	GCAATAAACCGGACCAACCT	50.0	50.0	57.9	58.5	1533-1608 bp	1.0	80.1