Hotspot

trnS-GCU-trnG-UCC

LSC · 7409-8257 · 849 bp

Back to Frankenia

Pi 0.00856

MarkerSeek score 67.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 7
pi: 0.00856
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	AATCAAACCGAAAGACCCCT	50.0	45.0	58.2	57.0	983-1014 bp	1.0	74.1
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	50.0	45.0	58.2	57.0	982-1013 bp	1.0	74.1
trnS-GCU-trnG-UCC_p3	TACGAATGACCCGTACAACG	AATCAAACCGAAAGACCCCT	50.0	45.0	57.7	57.0	955-986 bp	1.0	73.3
trnS-GCU-trnG-UCC_p4	TACGAATGACCCGTACAACG	ATCAAACCGAAAGACCCCTT	50.0	45.0	57.7	57.0	954-985 bp	1.0	73.3
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACCGAAAGACCCCTTAACTC	50.0	50.0	58.2	56.9	977-1008 bp	1.0	72.7