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trnK-UUU-rps16

LSC · 3856-4645 · 790 bp

Back to Frankenia

Pi 0.0133

MarkerSeek score 70.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 10
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GCGCTCAACCTACAGGAAC	50.0	57.9	58.2	58.5	847-883 bp	1.0	70.7
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GCGCTCAACCTACAGGAAC	50.0	57.9	58.2	58.5	846-882 bp	1.0	70.7
trnK-UUU-rps16_p3	TACCGTTGAGTTAGCAACCC	GCGCTCAACCTACAGGAAC	50.0	57.9	57.5	58.5	833-869 bp	1.0	68.4
trnK-UUU-rps16_p4	TCGCGGTCTCACAAACTTTA	GCGCTCAACCTACAGGAAC	45.0	57.9	57.5	58.5	932-968 bp	1.0	67.5
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	GCGCTCAACCTACAGGAAC	50.0	57.9	58.6	58.5	858-894 bp	1.0	67.4