Hotspot

psbL

LSC · 68426-68542 · 117 bp

Back to Frangula

Pi 0.00855

MarkerSeek score 57.40

Primer available yes

Label psbL

Feature Metadata

label_name: psbL
feature_type: gene
region: LSC
length_bp: 117
variable_sites: 1
pi: 0.00855
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL_p1	ATTTCATCAGGGGGTCATGC	CTGGCTGTACCTACCGTTTC	50.0	55.0	57.9	58.0	322 bp	1.0	79.6
psbL_p2	TTCATCAGGGGGTCATGCTA	CTGGCTGTACCTACCGTTTC	50.0	55.0	58.1	58.0	320 bp	1.0	79.3
psbL_p3	ATTTCATCAGGGGGTCATGC	ATGGTTAGCTGTTCACGGAC	50.0	50.0	57.9	57.9	341 bp	1.0	78.9
psbL_p4	TTCATCAGGGGGTCATGCTA	ATGGTTAGCTGTTCACGGAC	50.0	50.0	58.1	57.9	339 bp	1.0	78.6
psbL_p5	ATCAGGGGGTCATGCTAGAG	CTGGCTGTACCTACCGTTTC	55.0	55.0	58.3	58.0	317 bp	1.0	78.0