Hotspot

psbA

LSC · 643-1704 · 1062 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACACTAATACCAAAGCGAAGTC	TTATGTGCTTGGGAGTCCCT	40.9	50.0	56.5	58.3	1138 bp	1.0	49.1
psbA_p2	ACACTAATACCAAAGCGAAGTC	TTTATGTGCTTGGGAGTCCC	40.9	50.0	56.5	57.5	1139 bp	1.0	47.7
psbA_p3	ACACTAATACCAAAGCGAAGT	TTATGTGCTTGGGAGTCCCT	38.1	50.0	55.3	58.3	1138 bp	1.0	47.4
psbA_p4	ACACTAATACCAAAGCGAAGT	TTTATGTGCTTGGGAGTCCC	38.1	50.0	55.3	57.5	1139 bp	1.0	46.0
psbA_p5	ACACTAATACCAAAGCGAAGTC	ATTTATGTGCTTGGGAGTCCC	40.9	47.6	56.5	58.0	1140 bp	1.0	43.6