Hotspot

psaC

SSC · 123499-123744 · 246 bp

Back to Frangula

Pi 0.00407

MarkerSeek score 54.80

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TAGAGTATTTTGAGCGCGGG	CTGGGAAATCATTGATTCATCTGG	50.0	41.7	57.8	57.9	423 bp	1.0	45.8
psaC_p2	TAGAGTATTTTGAGCGCGGG	TGGGAAATCATTGATTCATCTGG	50.0	39.1	57.8	56.9	422 bp	1.0	45.5
psaC_p3	TAGAGTATTTTGAGCGCGGG	GGGAAATCATTGATTCATCTGGT	50.0	39.1	57.8	56.9	421 bp	1.0	45.5
psaC_p4	TAGAGTATTTTGAGCGCGGG	GGGAAATCATTGATTCATCTGGTG	50.0	41.7	57.8	58.2	421 bp	1.0	45.2
psaC_p5	CTTGGACCAGAAAAACCCGT	CTGGGAAATCATTGATTCATCTGG	50.0	41.7	58.3	57.9	452 bp	1.0	45.0