Hotspot

ccsA

SSC · 120619-121584 · 966 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGTAATTGAAAGACCTTCCTTGT	TTGTCAAGCCCGTTCTTCAA	34.8	45.0	56.0	58.0	1034 bp	1.0	40.0
ccsA_p2	TGTAATTGAAAGACCTTCCTTGT	TTTGTCAAGCCCGTTCTTCA	34.8	45.0	56.0	58.0	1035 bp	1.0	40.0
ccsA_p3	TGTAATTGAAAGACCTTCCTTGT	ATTCACGGGGTTCTCAATGG	34.8	50.0	56.0	57.9	1106 bp	1.0	40.0
ccsA_p4	TGTAATTGAAAGACCTTCCTTGT	GTGATTCACGGGGTTCTCAA	34.8	50.0	56.0	57.8	1109 bp	1.0	40.0
ccsA_p5	TGTAATTGAAAGACCTTCCTTGT	TGAAGTGATTCACGGGGTTC	34.8	50.0	56.0	57.8	1113 bp	1.0	40.0